Anti-RSK1 p90 (Phospho-T359/S363) RPS6KA1 Antibody

Product Name

Anti-RSK1 p90 (Phospho-T359/S363) RPS6KA1 Antibody

View all RSK1 Antibodies

SKU/Catalog Number

A01058T359S363-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-RSK1 p90 (Phospho-T359/S363) RPS6KA1 Antibody catalog # A01058T359S363-1. Tested in WB,ICC,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RSK1 p90 (Phospho-T359/S363) RPS6KA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01058T359S363-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Rsk-1/2/3/4 around the phosphorylation site of S221/227/S218/232.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01058T359S363-1 is reactive to RPS6KA1 in Human, Mouse, Rat

Applications

A01058T359S363-1 is guaranteed for IP, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

82.723kDa

Background of RSK1

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

Tao W., Nat. Genet. 21:177-181(1999).
Nishiyama Y., FEBS Lett. 459:159-165(1999).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of RPS6KA1 using Anti-RSK1 p90 (Phospho-T359/S363) RPS6KA1 Antibody (A01058T359S363-1).

ICC staining Phospho-RSK1 p90(T359+S363) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

Click image to see more details

Figure 1. Western blotting validation for Anti-RSK1 p90 (Phospho-T359/S363) RPS6KA1 Antibody A01058T359S363-1

Western blot analysis of Phospho-RSK1 p90(T359+S363) on different lysates using anti-Phospho-RSK1 p90(T359+S363) antibody at 1/1

Gene/Protein Information For RPS6KA1 (Source: Uniprot.org, NCBI)

Alternative Names

90kD, polypeptide 1); EC 2.7.11; EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1; HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPL1A; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK1; RSK-1; RSK1p90-RSK 1; S6K-alpha 1; S6K-alpha-1 RPS6KA1 HU-1, MAPKAPK1, MAPKAPK1A, RSK, RSK1, p90Rsk ribosomal protein S6 kinase A1 ribosomal protein S6 kinase alpha-1|90 kDa ribosomal protein S6 kinase 1|MAP kinase-activated protein kinase 1a|MAPK-activated protein kinase 1a|MAPKAP kinase 1a|MAPKAPK-1a|RSK-1|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|p90RSK1|p90S6K|ribosomal S6 kinase 1|ribosomal protein S6 kinase, 90kDa, polypeptide 1

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